Developing new compounds with bioactivity, especially against tumors, targeting G-quadruplexes is of great importance. In this direction, some new phthalimide-incorporating pyridine derivatives were synthesized using the phthalimido-acetophenone derivative 1 as a key precursor. Three-component cyclocondensation of compound 1 with various aryl aldehydes and ethyl cyanoacetate or malononitrile utili...