In the current study, thermo-tolerant fungal isolates were screened for their ability to metabolize L-methionine by L-methionine γ-lyase (MGL). Among 63 fungal isolates, fifteen isolates exhibited ability to MGL induction as evidenced by qualitative assay method using phenol red. Then, quantitative assay method indicates that fungal isolates coded as 26 & 37 were the most potent for MGL production...