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L-methionine γ-lyase from Thermo-tolerant fungi: Isolation, Identification of the potent producers, and Statistical Optimization of Production via Response surface methodology

Abstract
In the current study, thermo-tolerant fungal isolates were screened for their ability to metabolize L-methionine by L-methionine γ-lyase (MGL). Among 63 fungal isolates, fifteen isolates exhibited ability to MGL induction as evidenced by qualitative assay method using phenol red. Then, quantitative assay method indicates that fungal isolates coded as 26 & 37 were the most potent for MGL production...
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Keywords
Aspergillus fumigatus
Rhizomucor miehei
Response surface methodology
Central composite design
Mycelium
Biology
Methionine
Glycerol
Extracellular
Microbiology
Biochemistry
Food science
Chemistry
Enzyme
Botany
Chromatography
Lipase
Amino acid
Triacylglycerol lipase


pdf file

L-methionine γ-lyase from Thermo-tolerant fungi: Isolation, Identification of the potent producers, and Statistical Optimization of Production via Response surface methodology
pdf file

L-methionine γ-lyase from Thermo-tolerant fungi: Isolation, Identification of the potent producers, and Statistical Optimization of Production via Response surface methodology

Abstract
In the current study, thermo-tolerant fungal isolates were screened for their ability to metabolize L-methionine by L-methionine γ-lyase (MGL). Among 63 fungal isolates, fifteen isolates exhibited ability to MGL induction as evidenced by qualitative assay method using phenol red. Then, quantitative assay method indicates that fungal isolates coded as 26 & 37 were the most potent for MGL production...
View PDF
Keywords
Aspergillus fumigatus
Rhizomucor miehei
Response surface methodology
Central composite design
Mycelium
Biology
Methionine
Glycerol
Extracellular
Microbiology
Biochemistry
Food science
Chemistry
Enzyme
Botany
Chromatography
Lipase
Amino acid
Triacylglycerol lipase


pdf file